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Thank you for taking the time to visit the Court lab official recombineering website! Since launching our “old” site in 2011, we have had nearly 50,000 visitors! We hope you find this updated site even more useful. We aim to provide you with our most current information available on all things recombineering.

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What is recombineering?

Recombineering is in vivo genetic engineering, also known as homologous RECOMBInation-mediated genetic engiNEERING. “In vivo” is within a bacterial cell, usually E. coli or S. enterica, although recently recombineering has been done in other species, too. The genetic modifications are catalyzed by bacteriophage recombination proteins produced within the bacterium. Importantly, these phage functions are able to recombine DNAs containing short, 50 base, homologies. We typically use the bacteriophage λ Red recombination proteins for recombineering but other systems are available. For a more detailed look at recombineering and what can be done with it, look here: 4-17-09 02.tif

Background 

An example of optimized oligo recombination. Red sectors are the result of a galK amber mutation being corrected to WT with an oligo. In this experiment, 70% of the colonies contain the desired recombinant!

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