1. Thomason, L. C., Costantino, N., Shaw, D. V. & Court, D. L. (2007). Multicopy plasmid modification with phage λ Red recombineering. Plasmid 58, 148-158. Thomason-2007 Plasmid.pdf
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- FAQ 1: How much antibiotic should I use for chromosomal/BAC (single-copy) or plasmid (multi-copy) constructs?
- FAQ 2: What sequences do I use to make a drugR cassette for knock-outs?
- FAQ 3: What happens if I grow the recombineering cells at temperatures higher than 32°C?
- FAQ 4: Can’t I use an air shaker for the 42° induction?
- FAQ 5: Do the oligos I order need to be purified?
- FAQ 6: What happens if I induce for longer or shorter times?
- FAQ 7: Why don’t I get any recombinants?
- FAQ 8: I get tons of recombinants but when I analyze them, none are in the right place. What is wrong?
- FAQ 9: What conditions should I use for electroporation?
- FAQ 10: Why do my electroporations “pop” (arc)?
- FAQ 11: Is the amount of NaCl in the LB medium important?
- FAQ 12: How should I maintain the recombineering strains?
- FAQ 13: What do I do with the tube containing the “gel-like stuff” in it when you send me a strain or plasmid?
- FAQ 14: Why don’t I get the high frequencies that you report for ssDNA oligo recombineering?
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