banner image

General Recombineering


1.    1. Thomason, L. C., Sawitzke, J. A., Li, X., Costantino, N. & Court, D. L. (2014). Recombineering: genetic engineering in bacteria using homologous recombination. Curr Protoc Mol Biol 106, 1 16 1-1 16 39. CPMB 2014.pdf

2. Sawitzke, J. A., Thomason, L. C., Bubunenko, M., Li, X., Costantino, N. & Court, D. L. (2013). Recombineering: Using Drug Cassettes to Knock out Genes in vivo. Methods Enzymol. 533, 79-102. MIE dsDNA 2013.pdf

3.    Datta, S., Costantino, N., Zhou, X. & Court, D. L. (2008). Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phages. Proc. Natl. Acad. Sci. USA 105, 1626-1631. Datta-2008 PNAS .pdf

4.    Bubunenko, M., Baker, T. & Court, D. L. (2007). Essentiality of ribosomal and transcription antitermination proteins analyzed by systematic gene replacement in Escherichia coli. J Bacteriol 189, 2844-53. Bubunenko-2007 J. bact.pdf

5.    Datta, S., Costantino, N. & Court, D. L. (2006). A set of recombineering plasmids for gram-negative bacteria. Gene 379, 109-115. Datta-2006 Gene.pdf

6.    Yu, D., Sawitzke, J. A., Ellis, H. & Court, D. L. (2003). Recombineering with overlapping single-stranded DNA oligonucleotides: testing a recombination intermediate. Proc. Natl. Acad. Sci. USA 100, 7207-7212. PNAS2003-overlap.pdf

7.    Court, D. L., Swaminathan, S., Yu, D., Wilson, H., Baker, T., Bubunenko, M., Sawitzke, J. & Sharan, S. K. (2003). Mini-?: a tractable system for chromosome and BAC engineering. Gene 315, 63-69. Court-2003 Gene.pdf

8.    Yu, D., Ellis, H. M., Lee, E. C., Jenkins, N. A., Copeland, N. G. & Court, D. L. (2000). An efficient recombination system for chromosome engineering in Escherichia coli. Proc. Natl. Acad. Sci. USA 97, 5978-5983. Yu-2000 PNAS.pdf

9. Li, X., Thomason, L. C., Sawitzke, J. A., Costantino, N., & Court, D. L. (2013). Positive and negative selection using the tetA-sacB cassette: recombineering and P1 transduction in Escherichia coli. Nucleic Acids Res., 41, e204. Li-2013 NAR.pdf

NIH…Turning Discovery Into Health®
U.S. Department of Health and Human Services   |     National Institutes of Health  |   National Cancer Institute     |     USA.govHome  |  Contact  |  Policies  |  Accessibility  |  Viewing Files  |  FOIA