RECOMBINEERING
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Background info
Summary
What is recombineering?
Requirements for recombineering.
Discovery of recombineering
Recombineering with dsDNA.
in vivo cloning
Recombineering with ssDNA
Site Map
Optimizing recombineering with ssDNA
On the mechanism of ssDNA recombination
Moving recombineering to other systems
“Recombinase”-independent recombineering
FAQ
FAQ 1: How much antibiotic should I use for chromosomal/BAC (single-copy) or plasmid (multi-copy) constructs?
FAQ 2: What sequences do I use to make a drugR cassette for knock-outs?
FAQ 3: What happens if I grow the recombineering cells at temperatures higher than 32°C?
FAQ 4: Can’t I use an air shaker for the 42° induction?
FAQ 5: Do the oligos I order need to be purified?
FAQ 6: What happens if I induce for longer or shorter times?
FAQ 7: Why don’t I get any recombinants?
FAQ 8: I get tons of recombinants but when I analyze them, none are in the right place. What is wrong?
FAQ 9: What conditions should I use for electroporation?
FAQ 10: Why do my electroporations “pop” (arc)?
FAQ 11: Is the amount of NaCl in the LB medium important?
FAQ 12: How should I maintain the recombineering strains?
FAQ 13: What do I do with the tube containing the “gel-like stuff” in it when you send me a strain or plasmid?
FAQ 14: Why don’t I get the high frequencies that you report for ssDNA oligo recombineering?
Strains, Plasmids and Primers
Protocols
References
General Recombineering
High Throughput
ssDNA recombineering
Recombineering to BACs.
Recombineering to plasmids.
Recombineering to phage.
"Recombinase"-independent recombineering.
Recombineering reviews.
Links
Contact Form
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Oct 1, 2016
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