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FAQ 4: Can’t I use an air shaker for the 42° induction?

ANSWER 4: No, it will not work! It is critical to get the temperature up to 42° as quickly as possible. This cannot be achieved with an air shaker as the heat transfer is inefficient. If you must, you can use an air shaker for the 32° incubations but induction of the Red genes at 42° must be in a water bath, preferably with rapid (200-220rpm) shaking in a baffled flask. No induction = no recombination!