FAQ 11: Is the amount of NaCl in the LB medium important?

Answer 11: Yes! The LB we use contains: 5 g yeast extract, 10 g tryptone, and 5 g NaCl for 1 L media. Some recipes use 10 g NaCl. We have seen low viability of cells after electroporation when 10 g of NaCl is used.


 © RECOMBINEERING 2016