Answer 11: Yes! The LB we use contains: 5 g yeast extract, 10 g tryptone, and 5 g NaCl for 1 L media. Some recipes use 10 g NaCl. We have seen low viability of cells after electroporation when 10 g of NaCl is used.
Official website of the Court lab at the National Cancer Institute in Frederick, MD USA.
Answer 11: Yes! The LB we use contains: 5 g yeast extract, 10 g tryptone, and 5 g NaCl for 1 L media. Some recipes use 10 g NaCl. We have seen low viability of cells after electroporation when 10 g of NaCl is used.