FAQ 10: Why do my electroporations “pop” (arc)?

Answer 10: This is most often due to impurities or salts in the cells or the DNA but occasionally can be due to a cracked/defective cuvette or one that is too warm. We often store our cuvettes at -20° so they are ready to use. An arc will result in a low time constant and thus poor or no transformation. When this happens, set up a new electroporation with a fresh chilled cuvette and try again. If the cells or DNA are the problem, you may need to start over or try using less DNA. The DNA should always be diluted in ddH2O to keep salt to a minimum.


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