- Background info
- Summary
- What is recombineering?
- Requirements for recombineering.
- Discovery of recombineering
- Recombineering with dsDNA.
- in vivo cloning
- Recombineering with ssDNA
- Site Map
- Optimizing recombineering with ssDNA
- On the mechanism of ssDNA recombination
- Moving recombineering to other systems
- “Recombinase”-independent recombineering
- Frequently asked questions
- FAQ 1: How much antibiotic should I use for chromosomal/BAC (single-copy) or plasmid (multi-copy) constructs?
- FAQ 2: What sequences do I use to make a drugR cassette for knock-outs?
- FAQ 3: What happens if I grow the recombineering cells at temperatures higher than 32°C?
- FAQ 4: Can’t I use an air shaker for the 42° induction?
- FAQ 5: Do the oligos I order need to be purified?
- FAQ 6: What happens if I induce for longer or shorter times?
- FAQ 7: Why don’t I get any recombinants?
- FAQ 8: I get tons of recombinants but when I analyze them, none are in the right place. What is wrong?
- FAQ 9: What conditions should I use for electroporation?
- FAQ 10: Why do my electroporations “pop” (arc)?
- FAQ 11: Is the amount of NaCl in the LB medium important?
- FAQ 12: How should I maintain the recombineering strains?
- FAQ 13: What do I do with the tube containing the “gel-like stuff” in it when you send me a strain or plasmid?
- FAQ 14: Why don’t I get the high frequencies that you report for ssDNA oligo recombineering?
- Strains, Plasmids and Primers
- cassette primers 2016
- Protocols
- MIE review.pdf
- Oligo optimization.pdf
- Sharan-2009 Nature Protoc.pdf
- high throughput.pdf
- Thomason-2007 Plasmid.pdf
- Thomason-2009 Met MB.pdf
- preparing electrocompetent cells Lynno.pdf
- transformation by electroporation.pdf
- step-by-step ssDNA protocol.pdf
- Brief ssDNA protocol.pdf
- step-by-step knockout (dsDNA) protocol.pdf
- brief dsDNA.pdf
- checklist for plasmid recombineering.pdf
- Thomason-2007 P1 prot.pdf
- CPMB 2014.pdf
- References
- General Recombineering
- High Throughput
- ssDNA recombineering
- Recombineering to BACs.
- Recombineering to plasmids.
- Recombineering to phage.
- "Recombinase"-independent recombineering.
- Recombineering reviews.
- Oligo optimization.pdf
- Li-2003 Nuc acid res.pdf
- Costantino-2003 PNAS.pdf
- Ellis-2001 PNAS.pdf
- Thomason-2007 Plasmid.pdf
- Thomason-2009 Met MB.pdf
- Oppenheim-2004 Virol.pdf
- Swingle 2010.pdf
- Sharan-2009 Nature Protoc.pdf
- MIE review.pdf
- CurrentProtocols.pdf
- Courtetal2002.pdf
- Copeland-2001 Nat rev Genet.pdf
- Warming-2005 Nuc acid res.pdf
- Swaminathan-2001 Genesis.pdf
- Lee-2001 Genomics.pdf
- MIE 2013 ssDNA.pdf
- CPMB 2014.pdf
- Nucl. Acids Res.-2013-Li-e204
- Fu et al. RecET
- Useful links
- Court Lab
- Photo 2
- Contact Form
- pSIM5.pdf
- pSIM6.pdf
- pSIM7.pdf
- pSIM8.pdf
- pSIM9.pdf
- pSIM10.pdf
- pSIM17.pdf
- pSIM18.pdf
- pSIM19.pdf
- pSIM27.pdf
- pSIM29.pdf
- tet-sacB map.pdf
- pSIM5.txt.zip
- pSIM6.txt.zip
- pSIM7.txt.zip
- pSIM8.txt.zip
- pSIM9.txt.zip
- pSIM10.txt.zip
- pSIM17.txt.zip
- pSIM18.txt.zip
- pSIM19.txt.zip
- pSIM27.txt.zip
- pSIM29.txt.zip
- tet-sacB.txt.zip
- pSIM5.zip
- pSIM6.zip
- pSIM7.zip
- pSIM8.zip
- pSIM9.zip
- pSIM10.zip
- pSIM17.zip
- pSIM18.zip
- pSIM19.zip
- pSIM27.gcc.zip
- pSIM29.gcc.zip
- tet-sacB.gcs.zip
- Recombineering Strains.docx
- 4-17-09 02.tif
- lab sans Don.JPG
- Hhs.Gov
- Nih.Gov
- Cancer.Gov
- Usa.Gov
- Ccr.Cancer.Gov
