Moving recombineering to other systems

To date, potential recombineering systems have been identified in over 100 bacteria or bacteriophage that inhabit them (Blast search of Beta and RecT done February 2010), several of which have been cloned and studied in E. coli. The human herpes simplex virus contains analogs as well. These systems hold potential for organisms where the λ Red system does not function. As ssDNA recombination is better understood, less complex, and more efficient than dsDNA recombination, it should be attempted first when developing a new system and the optimized conditions developed in E. coli should be used.

 © RECOMBINEERING 2016