RECOMBINEERING - 2013
 



    WELCOME to the Court lab official recombineering website! Thank you for taking the time to visit us.  We hope you find it useful and value your comments on how we can improve it. We aim to provide you with our most current information available on all things recombineering (see the bottom of this page for the date when this site was last updated).



On this website you will find: recombineering protocols, strain lists, plasmid maps, plasmid sequences, and references available for downloading. We also include contact information to allow you to request reagents. If you are having problems with recombineering, we refer you first to the FAQ as we include information there to solve the most common problems we have heard about.


Happy recombineering!









Recombineering defined:


Recombineering is in vivo genetic engineering, also known as homologous RECOMBInation-mediated genetic engiNEERING. “In vivo” in this case, is within a bacterial cell, usually E. coli or S. enterica, but recently recombineering has been done in additional species. The genetic modifications are catalyzed by recombination proteins produced within the bacterium. Importantly, they are able to recombine DNAs containing short, 50 bp, homologies. We typically use the bacteriophage λ Red recombination proteins for recombineering but other systems are available. For a more detailed look at recombineering and what can be done with it, look here: Background


 


Last updated: October 22, 2013                            You are visitor number:


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An example of optimized oligo recombination. Red sectors are the result of a galK amber mutation being correct to WT with an oligo. In this experiment, 70% of the colonies had the desired recombinant!